This extensively revised new edition comprehensively reviews the
rise of clinical research informatics (CRI). It enables the
reader to develop a thorough understanding of how CRI has
developed and the evolving challenges facing the biomedical
informatician in the modern clinical research environment.
Emphasis is placed on the changing role of the consumer, and the
need to merge clinical care delivery and research as part of a
Electrical Manipulation of Cells provides an authoritative
and up-to-date review of the field, covering all the major
techniques in a single source. The book features broad coverage
that ranges from the mechanisms of action of external electrical
fields on biological material to the ways in which electrical
stimuli are employed to manipulate cells. Bringing together the
work of leading international authorities, the book covers membrane
breakdown, gene... more...
The standard protocols for the purification of all known
cytoskeleton proteins are presented in this manual. Proteins are
listed alphabetically and each protocol follows a common format.
Thus, the manual provides a quick and easy reference to all
relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear
flowchart, while the source of the protein, equipment and material
needed, a list of... more...
Fluorescence spectroscopy and its applications to the physical and
life sciences have evolved rapidly during the past decade. The
increased interest in fluorescence appears to be due to advances in
time resolution, methods of data analysis, and improved
instrumentation. With these advances, it is now practical to
perform time-resolved measurements with enough resolution to
compare the results with the structural and dynamic features of
mac- molecules,... more...
Determination of the protein sequence is as important today as it
was a half century ago, even though the techniques and purposes
have changed over time. Mass spectrometry has continued its recent
rapid development to find notable application in the
characterization of small amounts of protein, for example, in the
field of proteomics. The “traditional” chemical N-terminal
sequencing is still of great value in quality assurance of the
I started insect cell culture work in 1962, when T. D. C. Grace
reported the first establishment of invertebrate continuous cell
lines. He obtained grow ing cells from pupal ovaries of the
emperor gum moth, Antheraea euca lypti. At that time, I was trying
to obtain growing cells from leafhoppers. Grace's method could not
be applied directly to my culture because of the differences in
species, the size of the insects, and the tissue to be cul... more...
This volume covers methods for determination of autoantibodies in
rheumatic connective tissue diseases and organ-specific diseases.
Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known
This book expands on the previous volumes with new chapters
focusing on functional characterization of phage and their
proteins, and on the development of phage therapy by outlining
novel models. The chapters in this book cover molecular topics
such as PhageFISH for monitoring phage infections at single cell
level; the analysis of phage-host protein-protein interactions
using Strep-tag® II purifications; and also application driven... more...
...includes new information on molecular biology, bone marrow stem
cell transplantation, markers for thrombosis, and new learning aids
including chapter objectives, more case studies, color plates,
chapter review questions, etc.
This book is intended as a practical primer on the Pap test.
Using bold text, tables, and highlighted areas, this book offers
a user-friendly text on Pap test fundamentals so readers may find
specific information effortlessly. The book is a useful tool for
cytotechnologists and cytopathologists, as well as those in
training. Readers preparing for various proficiency and licensing
examinations will also find helpful information throughout.