I started insect cell culture work in 1962, when T. D. C. Grace
reported the first establishment of invertebrate continuous cell
lines. He obtained grow ing cells from pupal ovaries of the
emperor gum moth, Antheraea euca lypti. At that time, I was trying
to obtain growing cells from leafhoppers. Grace's method could not
be applied directly to my culture because of the differences in
species, the size of the insects, and the tissue to be cul... more...